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The researchers established a 5-compound (5C) protocol, consisting of SB431542, forskolin, DAPT (Notch inhibitor), IWP2 (Wnt inhibitor), and LDN193189 (BMP inhibitor) to suppress the expression of epithelial to mesenchymal transition marker genes (Xiang et al., 2019)

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Bacteriostatic water has become the standard in most research settings because it maintains sterility through repeated vial access, preserves peptide integrity without interference, and supports efficient multi-dose protocols