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The four degradation factors are heat fluctuation, light and UV exposure, repeated freeze-thaw cycles, and contamination from non-sterile technique

The OCR of spare respiratory capacity and its percentage ( Supplementary Table S3 ) were significantly higher in unstimulated cells compared to both types of stimulated PBMC

Lymphocyte membrane damage was analyzed from the activities of acetylcholinesterase and Na, K-ATPase

Methods Ethics statement Data analysis utilizing the TriNetX platform received approval from the Institutional Review Board of Chi-Mei Hospital (No: 11202-002), as well as approval from the institutional review boards of all participating hospitals